Thermo Fisher Scientific PDZ-RhoGEF Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

ARHGEF11 is a 1527 amino acid protein containing tandem dbl homology and pleckstrin homology domains, a PDZ domain, two proline-rich sequences, and a regulatory G protein-signaling (RGS) sequence.
It functions as a guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42, and acts as a GTPase-activating protein (GAP) for GNA12 and GNA13.
ARHGEF11 forms complexes with G proteins, stimulates neurite retraction through Rho-dependent signaling, and increases glutamate transport fourfold. It interacts with EAAT4, modulating uptake activity and distribution at glutamatergic synapses.
Overexpression induces actin cytoskeleton reorganization, membrane ruffling, and filopodia formation.
It is ubiquitously expressed in brain, lung, liver, kidney, and skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.