Thermo Fisher Scientific PDZ-RhoGEF Polyclonal Antibody
PDZ-RhoGEF 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 인간 ARHGEF11의 1472-1522 아미노산 영역을 면역원으로 제작. 액상 형태로 4°C 보관, 1년 권장 유효기간.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1472 and 1522 of human PDZ-RhoGEF (ARHGEF11, UniProt ID: O15085-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
ARHGEF11 is a 1527 amino acid protein containing tandem dbl homology and pleckstrin homology domains, a PDZ domain, two proline-rich sequences, and a regulatory G protein-signaling (RGS) sequence.
It functions as a guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42, and acts as a GTPase-activating protein (GAP) for GNA12 and GNA13.
ARHGEF11 forms complexes with G proteins, stimulates neurite retraction through Rho-dependent signaling, and increases glutamate transport fourfold. It interacts with EAAT4, modulating uptake activity and distribution at glutamatergic synapses.
Overexpression induces actin cytoskeleton reorganization, membrane ruffling, and filopodia formation.
It is ubiquitously expressed in brain, lung, liver, kidney, and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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