Thermo Fisher Scientific Firefly luciferase Monoclonal Antibody (LUC-Y)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

10 µg/mL

-

ELISA (ELISA)

1:500-1:5,000

-

Product Specifications

Species Reactivity

Luciferase

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LUC-Y

Immunogen

Recombinant luciferase expressed in E. Coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_934495

Product Specific Information

Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ~55-60 kDa using 1 µg of purified luciferase. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.

Target Information

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.