Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (AD1.1.10), HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000
• View 2 publications

ELISA

• Tested Dilution: 1:20–1:1,000
• View 3 publications

Product Specifications

Product Specific Information

Mouse anti-Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore valuable for detecting proteins containing histidine tags.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins lacking specific antibodies by fusing a known epitope to the recombinant protein.
His-tagged proteins can be purified via metal chelate chromatography due to the strong and selective affinity of histidine clusters to Ni²⁺ ions, enabling purification to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.