Thermo Fisher Scientific Huntingtin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-002 antibody has been validated for use in ELISA, Western Blot, immunofluorescence, and immunohistochemistry with human and mouse samples.

Neoepitope antibodies distinguish cleaved or processed protein fragments from full-length proteins by targeting specific cleavage sites.
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 cleaves at amino acids 513 and 552, caspase-2 at 552, and caspase-6 at 586.
Neo-specific antibody PA1-002 recognizes the 513-cleaved fragment of Huntingtin without detecting the full-length form.

Target Information

Huntingtin is a disease gene associated with Huntington’s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons.
HD results from expanded, unstable trinucleotide repeats in the huntingtin gene, producing a polyglutamine stretch in the protein.
It is an autosomal dominant disorder with mid-life onset, presenting with psychiatric symptoms, dementia, and involuntary movements (chorea), leading to death within 10–20 years.

The huntingtin locus spans approximately 180 kb with 67 exons. It is expressed as two alternatively polyadenylated transcripts (~13.7 kb and ~10.3 kb) with tissue-specific abundance.
The gene is essential for normal development and widely expressed in fetal and adult tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.