Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Super Bright 436, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 30 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocytes. Activation of both T and B cells rapidly upregulates CD69 surface expression, suggesting its role in lymphocyte development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes
• Recommended usage ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Titrate antibody for optimal performance

Fluorochrome Information:
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, a variable percentage of cells express CD69, which is involved in lymphocyte signal transduction and proliferation. CD69 expression is induced upon T lymphocyte activation and may also transmit signals in NK cells and platelets. Alternative splicing produces multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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