Thermo Fisher Scientific Mouse anti-Human IgE Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

1 µg/mL

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Secondary Antibody

Clone

E411 (5H2)

Immunogen

Immunoglobulin E from human serum.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931451

Target

IgE

Antibody Form

Whole Antibody

Product Specific Information

Mouse anti Human IgE antibody, clone E411 (5H2) recognizes human IgE heavy chain, a 428 amino acid protein bearing four Ig-like domains. Mouse anti Human IgE antibody, clone E411 binds to an epitope expressed on Cε3 domain.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.