Thermo Fisher Scientific TFF2 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific TFF2 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TFF-2. Anti-Human TFF-2-specific antibody was purified by affinity chromatography employing an immobilized Human TFF-2 matrix.

Sandwich ELISA:
To detect Human TFF-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TFF-2 (500-P312Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human TFF-2.

Western Blot:
To detect Human TFF-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TFF-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Trefoil peptides are protease-resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia. These peptides contain three intrachain disulfide bonds forming the trefoil motif (P-domain).
SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion. SP may act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.