Thermo Fisher Scientific IL-7 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 mg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-7. Anti-Rat IL-7-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IL-7 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-7 (500-P310) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-7.

Western Blot:
To detect Rat IL-7 by Western Blot analysis, use at a concentration of 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Rat IL-7 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P310BT-1MG is provided as 2 × 500 µg.

Target Information

Interleukin 7 (IL-7) is a hematopoietic cytokine constitutively produced by stromal cells from bone marrow and thymus. IL-7 signals through IL-7R to promote differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.