Thermo Fisher Scientific TFF2 Polyclonal Antibody, Biotin, PeproTech
Human TFF2 단백질을 인식하는 Biotin 표지 토끼 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 점막 치유 관련 TFF2 단백질 연구용으로 사용됩니다. 동결건조 형태로 -20°C 보관.
- 카탈로그번호
- 500-P312BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TFF2 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 12.0 kDa Recombinant Human TFF-2 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930339 |
Product Specific Information
AA Sequence of recombinant protein:
AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TFF-2. Anti-Human TFF-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human TFF-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TFF-2.
Western Blot:
To detect Human TFF-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible development reagents, the detection limit for Recombinant Human TFF-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P312BT-1MG will be provided as 2 × 500 µg.
Target Information
Trefoil peptides are protease-resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia.
These peptides contain three intrachain disulfide bonds forming the trefoil motif, or P-domain.
SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion.
SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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