Thermo Fisher Scientific TFF2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TFF-2. Anti-Human TFF-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human TFF-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TFF-2.

Western Blot:
To detect Human TFF-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible development reagents, the detection limit for Recombinant Human TFF-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P312BT-1MG will be provided as 2 × 500 µg.

Target Information

Trefoil peptides are protease-resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia.
These peptides contain three intrachain disulfide bonds forming the trefoil motif, or P-domain.
SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion.
SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.