Thermo Fisher Scientific Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (2E3A3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1 µg/mL

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2E3A3

Immunogen

A synthetic phosphorylated peptide around S473 of human AKT1 (NP_0051542) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT Pan,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:471-475|473,antigenLength:480,antigenImageFileName:MA5-38243_AKT_Pan_P31749-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38243_AKT_Pan_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT Pan,uniProtId:P31751-1,ncbiNodeId:9606,antigenRange:472-476|474,antigenLength:481,antigenImageFileName:MA5-38243_AKT_Pan_P31751-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38243_AKT_Pan_P31751-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:AKT Pan,uniProtId:Q9Y243-1,ncbiNodeId:9606,antigenRange:470-474|472,antigenLength:479,antigenImageFileName:MA5-38243_AKT_Pan_Q9Y243-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38243_AKT_Pan_Q9Y243-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2898159

Product Specific Information

Positive Samples: Jurkat, NIH/3T3, C6

Immunogen sequence: QFSYS

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.