Thermo Fisher Scientific MTCO1 Polyclonal Antibody
Rabbit polyclonal antibody targeting MTCO1, reactive with human, mouse, and rat. Validated for Western blot (1:500–1:1,000). Affinity-purified with >95% purity. Supplied as liquid, 1 mg/mL in PBS with 50% glycerol. Suitable for mitochondrial protein re...
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Applications
- Western Blot (WB): 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 433–445 of Mouse MTCO1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4 °C (short term); for long term, store at −20 °C, avoiding freeze/thaw cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2718926 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum using an epitope-specific immunogen. Purity is > 95% (by SDS-PAGE).
Target Information
Cytochrome c oxidase subunit I (CO1 or MTCO1) is one of three mitochondrial DNA–encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV, located in the mitochondrial inner membrane, is the terminal enzyme of the electron transport chain in oxidative phosphorylation. It consists of 13 polypeptides—three encoded by mtDNA and ten by nuclear DNA.
The enzyme contains four redox centers (two hemes and two copper centers). In mitochondrial Complex IV, hemes a and a3 and copper CuB are bound to subunit I. Mutations in the COX subunit I gene can affect enzyme function and mitochondrial respiratory efficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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