Thermo Fisher Scientific CD3e Monoclonal Antibody (eBio500A2 (500A2)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (eBio500A2 (500A2)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody can partially cross-block the 17A2 and 145-2C11 antibodies, indicating overlapping epitopes.

Applications Tested:
This antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at ≤0.125 µg per test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Fluorochrome Information:
PerCP-eFluor™ 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue Laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable alternative)
  PerCP-eFluor™ 710 conjugated antibodies are stable in 2% formaldehyde overnight at 4°C.
  Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating TCR surface expression. CD3 gamma, delta, epsilon, and zeta subunits are structurally related immunoglobulin superfamily members encoded on chromosome 11. These subunits associate with signal transduction molecules via tyrosine-based motifs. CD3 plays roles in TCR-induced growth arrest, cell survival, and proliferation.
CD3 antigen is found on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum. Not expressed on B or NK cells. CD3 gene defects are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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