Thermo Fisher Scientific DFFB Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• In Western blot applications, this antibody detects a band at approximately 40 kDa.
• Suggested positive control: mouse lung tissue lysate.
• PA5-19892 can be used with blocking peptide PEP-0020.

Target Information

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis, a programmed cell death process, removes toxic or unnecessary cells during mammalian development and can be induced by various stimuli such as tumor necrosis factor family death factors, UV irradiation, anticancer drugs, and deprivation of survival factors.
During apoptosis, cells and nuclei shrink and fragment, and chromosomal DNA is degraded into nucleosomal units. CAD, activated by caspases, forms a complex with its inhibitor, ICAD (inhibitor of CAD). ICAD assists in proper folding of CAD and inhibits its DNase activity. When caspase is activated, ICAD is cleaved, releasing CAD to enter the nucleus and cleave chromosomal DNA.
The activation of caspases is regulated by the balance between pro- and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Fourteen members of the caspase family have been identified, seven of which mediate apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.