Thermo Fisher Scientific DFFB Polyclonal Antibody
DFFB 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB 및 IHC에 적합합니다. Mouse와 Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 비결합형 액상 형태로 1.0 mg/mL 농도이며, 냉장 또는 냉동 보관이 가능합니다.
- 카탈로그번호
- PA519891
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 17 amino acids near the center of murine CAD |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154991 |
Product Specific Information
- In Western blot applications, this antibody detects a band at approximately 40 kDa.
- Suggested positive control: mouse kidney tissue lysate.
- PA5-19891 can be used with blocking peptide PEP-0019.
Target Information
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis, a programmed cell death process that removes damaged or unnecessary cells during mammalian development, can be induced by various stimuli such as tumor necrosis factor family death factors, UV irradiation, anticancer drugs, and deprivation of survival factors.
During apoptosis, cells and nuclei undergo shrinkage and fragmentation, and chromosomal DNA is degraded into nucleosomal units. CAD, activated by caspases, exists as a complex with its inhibitor ICAD (Inhibitor of CAD). ICAD assists in proper folding of CAD and inhibits its DNase activity. Upon activation, caspases cleave ICAD, releasing CAD to enter the nucleus and cleave chromosomal DNA.
Activation of caspases is regulated by the balance between pro- and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Fourteen members of the caspase family have been identified, with seven mediating apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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