Thermo Fisher Scientific CD83 Monoclonal Antibody (HB15e), PE, eBioscience

Thermo Fisher Scientific CD83 Monoclonal Antibody (HB15e), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 17 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein belonging to the Ig superfamily. CD83 is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells, as well as some proliferating lymphocytes and human cell lines.
While the function of CD83 remains unclear, it serves as a useful marker for mature human blood dendritic cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended use at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD83 is a 40–45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 after stimulation with agents such as Con A or PHA.
In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by germinal center cells. It is also expressed by Langerhans cells in the skin.

The CD83 antigen is a 186-amino-acid single-chain glycoprotein, part of the immunoglobulin superfamily, consisting of:

• Extracellular V-type Ig-like domain
• Transmembrane region
• Short (40 amino acid) cytoplasmic tail

CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of its core protein.
CD83⁺ cells have a unique surface phenotype distinct from T, B, NK, or myelomonocytic lineages, and co-express high levels of MHC class II molecules along with T cell (CD2, CD5), B cell (CD40, CD78), and myeloid markers (CD13, CD33, CD36).
Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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