Thermo Fisher Scientific Phospho-c-Fos (Thr232) Polyclonal Antibody
Phospho-c-Fos (Thr232) 특이적 폴리클로날 항체로, WB, IHC, ICC, ELISA 등 다양한 응용 가능. Mouse 반응성, Rabbit IgG 호스트. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포 신호전달 및 인산화 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from mouse c-Fos around the phosphorylation site of Thr232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene on chromosome 14.
c-Fos forms a heterodimer with c-Jun to create the Activator Protein-1 (AP-1) transcription factor, which regulates genes involved in cellular signaling, proliferation, and differentiation.
Phosphorylation of c-Fos occurs at multiple sites in response to stimuli such as growth factors and insulin. The best-known kinase for phosphorylation at Threonine 232 is ERK MAPK, which controls nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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