Thermo Fisher Scientific Phospho-c-Fos (Thr325) Polyclonal Antibody
Phospho-c-Fos (Thr325) 폴리클로날 항체로, 인간, 마우스, 랫트 시료에 사용 가능. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합. Protein A로 정제된 고순도 항체로, 세포 신호전달 및 인산화 연구에 유용. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos forms a heterodimer with c-Jun to create the Activator Protein-1 (AP-1) transcription factor, which regulates genes involved in signal transduction, cell proliferation, and differentiation.
It is phosphorylated at multiple sites by various stimuli, including growth factors and insulin. The ERK MAPK kinase is known to phosphorylate c-Fos at Threonine 232, regulating its nuclear localization and transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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