
Thermo Fisher Scientific PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Recombinant Mouse Monoclonal Antibody (rPDL1, 4772)
인간 PD-L1 단백질을 인식하는 재조합 마우스 단클론 항체입니다. IHC(P) 실험에 적합하며, HEK293 세포에서 발현되었습니다. Protein A/G로 정제되었고, PBS 완충액에 0.05% BSA가 포함되어 있습니다. 암 면역치료 연구용으로 활용됩니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rPDL1, 4772 |
| Immunogen | KLH-conjugated linear peptide corresponding to human PD-L1 (amino acids 200–290). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Programmed death receptor ligand 1 (PD-L1, also known as B7-H1) is a B7 family member that binds to programmed death receptor 1 (PD-1), negatively regulating T-cell receptor (TCR) signaling. PD-L1 engagement decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and is upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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