Thermo Fisher Scientific PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Recombinant Mouse Monoclonal Antibody (rPDL1, 4773)
PD-L1(CD274) 표적 리컴비넌트 마우스 모노클로날 항체로 암 면역치료 연구용. Western blot과 IHC(Paraffin)에서 1–2 µg/mL 농도로 사용 가능. HEK293 세포 발현, Protein A/G 정제, PBS 완충액에 보관. 4°C 저장, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rPDL1, 4773 |
| Immunogen | Recombinant fragment (around aa 39–191) of human CD274 (PD-L1) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described member of the B7 family. Its receptor, programmed death receptor 1 (PD-1), negatively regulates T-cell receptor (TCR) signaling. Upon ligation, PD-L1 decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated upon IFN-gamma stimulation, indicating its potential role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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