Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (intracellular staining)
• Tested Dilution: 0.125 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 737
• Brilliant Violet 421, 480, 605
• eFluor 450
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). iNOS catalyzes nitric oxide formation from L-arginine via an NADPH- and oxygen-dependent mechanism. NOS2 expression is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α, and is found in M1 macrophages and myeloid-derived suppressor cells.

• Reported Applications: Intracellular staining for flow cytometric analysis
• Tested Applications: Intracellular staining of mouse thioglycolate-elicited peritoneal exudate cells using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
• Use ≤ 0.125 µg/test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Carefully titrate for optimal performance

Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, optimized for cytometers equipped with a 405 nm violet laser.

Important Notes:

• Use Super Bright or Brilliant Stain Buffer to minimize non-specific polymer interactions.
• Protect vial and stained samples from light (photo-induced oxidation sensitivity).
• Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in dark.
• Not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

iNOS (Inducible Nitric Oxide Synthase)
iNOS produces nitric oxide (NO), a gaseous free radical mediating vasorelaxation, neurotransmission, and cytotoxicity. It is inducible by lipopolysaccharides and cytokines (IFN, TNF, IL-1, IL-2). Found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells. iNOS activity increases after cytokine induction and remains elevated for extended periods. Dysfunction is associated with achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지