Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet 786, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• Alexa Fluor Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and M), but absent in resting cells (G0). Ki-67 serves as a marker for determining the growth fraction of cell populations and is used in tumor cell studies to evaluate proliferation index.

This antibody also recognizes human, non-human primate, and canine Ki-67.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended use ≤1.0 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration for best performance.

Fluorochrome Information:
Brilliant Violet™ 786 (BV786) emits at 786 nm and is excited by a 405 nm violet laser. Ensure the cytometer is compatible with this fluorochrome.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle, but absent in G0. It associates with euchromatin and heterochromatin regions and is used as a proliferation marker in neoplastic and neuronal studies. Ki-67 index correlates with tumor grade and proliferation rate, providing valuable prognostic information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.