Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Brilliant Violet 786

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is upregulated by the presence of IgE. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells at 5 µL (0.5 µg)/test.

Brilliant Violet™ 786 (BV786) emits at 786 nm, designed for use with violet (405 nm) laser-equipped cytometers. Ensure instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, employ Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) at 4°C in the dark. BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

FceR1 (FcεRIα) is a subunit of the high-affinity receptor for IgE. It forms a tetrameric complex with one α, one β, and two γ subunits. The FcεRI complex triggers allergic responses through IgE receptor activation, leading to release of histamine and other mediators responsible for allergy symptoms such as hay fever and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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