Thermo Fisher Scientific STK29 Polyclonal Antibody
STK29(BRSK2) 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제됨. 인간 및 마우스 반응성, 액상 형태로 제공되며, 냉장 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 23 amino acid peptide from near the carboxy terminus of human BRSK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154173 |
Product Specific Information
- Suggested positive control: Human brain tissue lysate
- PA5-20358 can be used with blocking peptide PEP-0478.
Target Information
BRSK2 (Brain-selective serine/threonine kinase 2) is primarily expressed in the brain, with weaker expression in testis and pancreas.
BRSK2 phosphorylates WEE1A, CDC25C, and CDC25B in vitro.
DNA damage induced by UV irradiation or methyl methane sulfonate enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes its translocation from the cytoplasm to the nucleus.
Overexpression of BRSK2 in HeLa cells increases phosphorylation of CDC25C and induces G2/M arrest.
Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest, suggesting BRSK2 functions as a checkpoint kinase upon DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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