Thermo Fisher Scientific WT1 Monoclonal Antibody (6F-H2)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:400

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2-10 µg/mL lysate

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6F-H2

Immunogen

Recombinant protein corresponding to residues 1-181 of human WT1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WT1,uniProtId:P19544-1,ncbiNodeId:9606,antigenRange:1-181,antigenLength:449,antigenImageFileName:MA1-46028_WT1_P19544-1_House_mouse.svg,antigenImageFileNamePDP:MA1-46028_WT1_P19544-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_962464

Product Specific Information

MA1-46028 detects an epitope within residues 1-84.

Target Information

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.