Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience
CD171(L1CAM)을 인식하는 eBio5G3(5G3) 비오틴 결합 단클론 항체로, 인간 시료에 반응합니다. Flow Cytometry, WB, IHC 등 다양한 응용에 사용 가능하며, 0.5 µg/test로 최적화된 성능을 제공합니다. 신경세포 접착 및 종양 진행 연구에 적합합니다.
- 카탈로그번호
- 13-1719-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience
Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (PFA fixed) | - |
| Immunohistochemistry (Frozen) | Assay-Dependent |
| Flow Cytometry (Flow) | 0.5 µg/test |
| ELISA | - |
| Immunoprecipitation (IP) | - |
| Affinity Purification (AP) | - |
| In vitro Assay (IV) | - |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5G3 (5G3) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043814 |
Additional Formats
- Unconjugated
- APC
- PE
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody eBio5G3 recognizes CD171 (L1CAM), a neural cell adhesion molecule belonging to the Ig superfamily. CD171 contains six extracellular Ig domains and five fibronectin type III-like repeats. It mediates cell-cell interactions involved in neuronal myelination, neurite outgrowth, and regeneration.
CD171 is expressed on monocytes, mature monocytic-derived and follicular dendritic cells, a minor subset of lymphocytes, neuronal tissue, and certain tumor cell lines. Tumor expression of CD171 is associated with progression. The epitope recognized by eBio5G3 is located in the amino-terminal Ig-like domain.
Applications Reported:
Flow cytometry, Western blotting, and immunohistology staining of frozen tissue sections.
Applications Tested:
Validated by flow cytometric analysis of Panc-1 cells. Recommended usage ≤ 0.5 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ per test; titration is advised for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
L1CAM/CD171 is an axonal glycoprotein of the immunoglobulin supergene family. Its ectodomain consists of multiple Ig-like and fibronectin type III domains, linked via a transmembrane sequence to a conserved cytoplasmic domain. This molecule is critical for nervous system development, including neuronal migration and differentiation.
Mutations in L1CAM cause X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus). Neuron-specific alternative splicing is functionally significant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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