Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The HIP1 monoclonal antibody reacts with human CD42b, a 135 kDa transmembrane protein also known as platelet gpIb.
Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of human platelets at 5 µL (0.5 µg) per test.
A test is defined as the amount of antibody required to stain a cell sample in 100 µL volume. Typical cell numbers range from 10⁵ to 10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample for antibody cocktails or single-color controls.

Technology:
NovaFluor conjugates utilize Phiton™ technology, employing novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

CD42b is a platelet activation marker involved in coagulation and adhesion processes. It interacts with extracellular matrix and adhesion molecules and forms part of the GPIb-IX-V receptor complex, essential for platelet-rich thrombus formation.
CD42b binds to von Willebrand factor (VWF), thrombin, coagulation factors XI and XII, and other adhesion molecules.
It is expressed on platelets and megakaryocytes in bone marrow.
Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지