Takara Tetracycline-inducible miRNA

The Mir-X inducible miRNA red and green systems exploit key elements of our Tet-On Advanced Inducible Expression System to provide controlled co-expression of your selected microRNA and a bright red or green fluorescent protein marker.

The Mir-X inducible miRNA red and green systems exploit key elements of our Tet-On Advanced Inducible Expression System to provide controlled co-expression of your selected microRNA and a bright red or green fluorescent protein marker.

How it works

Your microRNA sequence is embedded in the 3’ UTR of a marker protein mRNA, which is expressed from an inducible miRNA expression vector (pmRi-mCherry or pmRi-ZsGreen1). When cells expressing the Tet-On Advanced transactivator are transfected with the pmRi-mCherry or pmRi-ZsGreen1 vector, high levels of your inducible microRNA and either the mCherry or ZsGreen1 proteins are expressed in response to doxycycline (Dox). Once Dox is added to the cell culture medium, miRNA induction is very rapid and effective.

We used modified _Metridia_-secreted luciferase genes containing miRNA target sequences (RNAiMonitor) to measure the effects of miR-1 and miR-9 miRNA induction in MCF7 Tet-On Advanced cells. Inducing miR-1 and miR-9 expression in these cells with Dox resulted in >90% knockdown of luciferase activity compared to controls.

Red and green complete systems

The complete Mir-X inducible miRNA systems provide the pTet-On Advanced Vector for expressing the Dox-responsive transactivator; an inducible miRNA expression vector (either pmRi-mCherry or pmRi-ZsGreen1, also available separately); two linear selectable markers (hygromycin and puromycin) for cotransfecting with the pmRi vectors in order to establish stable cell lines; and tetracycline-free Tet System Approved FBS (serum) for ensuring the full activity of Tet-based expression systems.

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Overview

• Track your inducible miRNA expression with fluorescent protein markers
• High fold-induction and low basal expression

More Information

Applications

• Tetracycline-inducible miRNA expression in mammalian cells

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.