Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor 700, eBioscience

Applications

Tested Dilution

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available upon request

Product Specific Information

The eBio299Arm monoclonal antibody is an isotype control immunoglobulin suitable for use in flow cytometric analysis.
It has been tested on mouse and human hematopoietic cells. Use the isotype control at the same concentration as the experimental antibody.

Fluorochrome Information:
Alexa Fluor™ 700 emits at 723 nm and can be excited with a 633 nm red laser.
Typical detection setup: 685 LP mirror and 710/20 filter.
Ensure that the instrument supports this fluorochrome.

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Isotype control antibodies help distinguish non-specific background signal from specific antibody signal.
They are essential in applications such as:

• Flow cytometry (most common)
• Chromatin immunoprecipitation (ChIP)
• Immunohistochemistry
• Gel shift assays

Isotype controls should match the species and isotype of the primary antibody and, when applicable, use the same conjugated label for accurate comparison.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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