Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Alexa Fluor 700, eBioscience
인간 CD19 항원을 인식하는 HIB19 단클론 항체로, Alexa Fluor 700 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, B세포 발현 연구에 활용됩니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 56-0199-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 31 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043819 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow series
- eFluor 450, 506
- FITC, PE, PE-Cyanine5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series
- NovaFluor Blue, Red, Violet, Yellow, UV series
- Request custom conjugation
Product Specific Information
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except on terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR complex. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test in 100 µL final volume, with cell numbers ranging from 10⁵–10⁸ cells/test.
Fluorochrome Details:
Alexa Fluor® 700 emits at 723 nm and is excited with the red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Filtered post-manufacturing with 0.2 µm filtration.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and some myeloid leukemia cells. CD19 serves as a signal-amplifying co-receptor for the B cell receptor (BCR) and regulates normal and malignant B cell proliferation. Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.