
Thermo Fisher Scientific Kappa Light Chain Recombinant Mouse Monoclonal Antibody (rKLC264)
인간 Kappa Light Chain을 인식하는 마우스 유래 재조합 단일클론 항체. IHC(P) 및 Peptide Array에 사용 가능. 고순도의 Protein A/G 정제 항체로 4°C에서 보관. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKLC264 |
| Immunogen | Recombinant human Ig kappa chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Immunoglobulin classes share a basic structure of two heavy chains and two light chains (kappa and lambda, each ~22.5 kDa).
Kappa and lambda light chains have variable and constant regions, which differ antigenically.
The kappa to lambda ratio is approximately 70:30.
Antibodies to the kappa light chain are useful for identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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