Thermo Fisher Scientific alpha Internexin Monoclonal Antibody (1D2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (IHC)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:250-1:5,000

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D2

Immunogen

Recombinant rat alpha-internexin expressed in and purified from E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein G

Storage buffer

PBS

Contains

10mM sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10981235

Product Specific Information

In Western blot, a band at ~66 kDa is detected. The epitope recognized by MA1-4709 (C-terminal non-helical extension of the protein) is unusually resistant to aldehyde fixation, so formalin fixation of histological sections is recommended.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.