Thermo Fisher Scientific NSE Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are damaged.

NSE is one of three mammalian enolases:

• ENO1 (α-subunit): expressed in most tissues
• ENO2 (γ-subunit, NSE): expressed in neurons and neuroendocrine cells
• ENO3 (β-subunit): expressed in muscle

Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Because neurons require high energy, they are rich in glycolytic enzymes such as GAPDH and NSE. Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells.

Release of NSE from damaged neurons into CSF and blood serves as a biomarker of neuronal injury. Clinically, NSE is a sensitive marker for neuronal damage in disorders such as stroke, hypoxic brain injury, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also observed in certain neoplasias including pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.