Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Red 660, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Red 660, eBioscience

Applications

• Flow Cytometry

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer.
CD8 binds to MHC class I and, through association with tyrosine kinase p56lck, plays a role in T cell development and activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤ 0.5 µg/test)

A test corresponds to staining a 100 µL cell sample (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Compatibility Notice:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

Blocking Buffer Recommendation:
Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample to antibody cocktails or single-color controls.

NovaFluor conjugates utilize Phiton™ technology, enabling engineered fluorescent signatures with minimized spillover and spread.

Excitation: 637 nm
Emission: 659 nm
Laser: 633–640 nm (Red)

Target Information

CD8 is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for T cell signal transduction, differentiation, and activation.
Predominantly expressed on T cells as CD8αβ heterodimers, CD8 acts as a co-receptor for MHC-I molecules, facilitating cytotoxic T lymphocyte (CTL) activation and pathogen clearance.
CD8α is also found on NK cells, monocytes, and dendritic cells as CD8αα homodimers.
Activation triggers Lck recruitment, lymphokine production, and CTL-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures.

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