Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Violet 800, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Violet 800, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: ≤ 0.4 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV, Brilliant Violet, eFluor, FITC
• NovaFluor Blue, Red, Yellow, Violet variants
• PE, PE-Cyanine, PerCP-Cyanine, Super Bright series
• Request custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer.

• Expressed on thymocytes, mature T cells, γδ T cells, intestinal intraepithelial lymphocytes, and dendritic cells.
• CD8 binds MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤ 0.4 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Always use CellBlox Plus Blocking Buffer for optimal staining. Add 5 µL per sample (10³–10⁸ cells) before combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

• Excitation: 408 nm
• Emission: 800 nm
• Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family.

• Functions as a co-receptor with TCR for MHC class I molecules.
• Expressed on T cells, NK cells, monocytes, and dendritic cells.
• CD8α chain essential for MHC-I binding.
• Activation triggers Lck recruitment, lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
• Differentiation of naive CD8⁺ T cells into CTLs enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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