Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution ≤ 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450–660)
• FITC, PE, PE-Cyanine series, PerCP series
• Super Bright series (436–780)
• NovaFluor Blue, Violet, Yellow series
• Request custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer.

• CD8αβ: Expressed on thymocytes and mature αβ TCR T cells
• CD8αα: Found on γδ TCR T cells, intestinal IELs, and dendritic cells
  CD8 binds MHC class I and associates with p56lck, contributing to T cell development and activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes; ≤ 0.5 µg/test (100 µL final volume). Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample to antibody cocktails or single-color controls.

Technology:
NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescent signatures, minimizing spillover and spread.

Spectral Properties:

• Excitation: 639 nm
• Emission: 710 nm
• Laser: 633–640 nm (Red)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein functioning as a co-receptor with TCR for MHC class I molecules.

• Expressed on T cells, NK cells, monocytes, and dendritic cells
• Exists as CD8αβ heterodimer or CD8αα homodimer
• Binding to MHC-I triggers Lck recruitment and CTL activation
• CTLs play a key role in pathogen and tumor cell clearance
• Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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