Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Red 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506, 615, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• NovaFluor Blue, Red, Violet, Yellow series
• Functional Grade
• Custom conjugation available

Product Specific Information

Description:
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). It is found on thymocytes, mature αβ TCR T cells, γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells. CD8 binds to MHC class I and interacts with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤ 0.5 µg per test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Optimize antibody concentration for best performance

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Use CellBlox Blocking Buffer (5 µL per sample) when staining with NovaFluor conjugates, including single-color controls.
• Excitation: 639 nm; Emission: 700 nm; Laser: 633–640 nm (Red Laser).
• NovaFluor conjugates utilize Phiton™ technology, offering engineered fluorescent signatures with reduced spillover and spread.

Target Information

CD8 (Cluster of Differentiation 8):
A type I transmembrane glycoprotein of the immunoglobulin receptor family involved in T cell signal transduction, differentiation, and activation.

• Expressed mainly on T cells as CD8αβ heterodimers, functioning as co-receptors for MHC class I molecules.
• CD8α homodimers are also found on subsets of T cells, NK cells, monocytes, and dendritic cells.
• Engagement with MHC-I/peptide complexes recruits Lck kinase, leading to cytokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
• Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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