Thermo Fisher Scientific B7-H4 Monoclonal Antibody (H74), APC, eBioscience

Thermo Fisher Scientific B7-H4 Monoclonal Antibody (H74), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The H74 monoclonal antibody was generated to react with human B7-S1, also known as B7-H4 and B7x. Cross-reactivity to other proteins has not been determined.
B7S1 is a newly discovered member of the B7 family and may influence T-cell costimulatory regulation depending on B-cell activation status.
BTLA has been reported as a counter receptor for B7S1, but further studies are needed to confirm the ligand.
H74 stains human B7-H4 transfected cells but not peripheral blood cells; expression pattern remains incompletely characterized.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested using human B7-H4-transfected cells at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume (typically 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

B7-H4 (B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity.
Although its receptor is unknown, it delivers inhibitory signals to T-cells, suppressing proliferation, cell cycle progression, and IL-2 production.
B7-H4 deficient mice show minimal effects, suggesting a fine-tuning role in T-cell regulation.
It is expressed on activated T-cells, B-cells, monocytes, and dendritic cells.
Aberrant expression is linked to lung, breast, and ovarian cancers.
Overexpression may help tumor cells evade immune detection, making B7-H4 a potential biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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