
Thermo Fisher Scientific CD272 (BTLA) Monoclonal Antibody (8F4), APC, eBioscience
마우스 BTLA 단백질을 인식하는 APC 결합 단클론 항체로, Flow Cytometry에 최적화됨. 8F4 클론으로 제작되어 높은 특이성과 재현성을 제공. 651/660 nm 형광 특성, 4°C 보관 및 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8F4 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10670228 |
Additional Formats
Product Specific Information
The BTLA antibody (clone 8F4) reacts with mouse BTLA (B and T lymphocyte attenuator) from BALB/c and C57Bl/6 strains.
BTLA is expressed on peripheral lymphocytes, splenic macrophages, developing B cells in bone marrow, developing T cells in thymus, and mature dendritic cells. It functions as a negative regulator of activation and function in various hematopoietic cells.
The anti-mouse BTLA clone 6F7 stains CD4+ and CD8+ SP thymocytes, while clones 8F4, 3F9.D12, 6G3, and 6H6 do not. Despite this, these clones show similar staining in splenocytes and bone marrow cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes. Use ≤0.125 µg per test (100 µL final volume; 10⁵–10⁸ cells/test). Optimize titration for best performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BTLA (B and T-lymphocyte attenuator) is a co-inhibitory receptor of the CD28 superfamily. It negatively regulates lymphocyte activation during immune responses.
BTLA is constitutively expressed on CD4+ and CD8+ T cells, decreasing upon activation, remaining on Th1 but not Th2 cells.
It interacts with HVEM (Herpesvirus Entry Mediator), a TNF receptor superfamily member, to regulate lymphocyte activation and homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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