
Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), PE, eBioscience
마우스 Ly-6C 단백질을 인식하는 단클론 항체로, PE 형광 표지되어 흐름세포분석(Flow Cytometry)에 최적화됨. HK1.4 클론으로 다양한 마우스 계통에서 CD4/CD8 발현 차이를 인식. 0.125 µg/test로 사용 가능하며, 4°C 암소 보관. 연구용 전용 제품.
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Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 102 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HK1.4 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10805389 |
Available Formats
- Alexa Fluor 488
- APC
- APC-eFluor 780
- eFluor 450
- Functional Grade
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Request custom conjugation
Product Specific Information
The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family.
Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and subsets of lymphocytes.
Expression varies among mouse strains:
- Ly6C.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
- Ly6C.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells
In vitro, HK1.4 antibody can enhance proliferation and stimulate cytokine release.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤0.125 µg/test, 100 µL final volume)
Recommended to titrate for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Granulocytes are white blood cells containing granules in their cytoplasm, also known as polymorphonuclear leukocytes (PMN/PML) due to their multi-lobed nuclei.
Neutrophil granulocytes are the most abundant type.
Granulocytes are released from bone marrow under regulation by complement proteins.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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