Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Violet 605, eBioscience™

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–711)
• eFluor 450, 506
• FITC
• NovaFluor Blue/Red/Yellow series
• PE, PE-Cyanine5/5.5/7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. It plays a critical role in lymphocyte activation via tyrosine phosphatase activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted for use at 5 µL (0.5 µg)/test with human peripheral blood cells
• Laser Compatibility: Violet laser (405 nm)
• Emission: 605 nm

When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Super Bright™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It regulates T- and B-cell receptor signaling, cytokine receptor signaling, and integrin-mediated pathways. Multiple isoforms arise from alternative splicing and differ in extracellular domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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