Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450–660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, 5.5, 7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The RA3-6B2 monoclonal antibody reacts with the exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is primarily expressed by B cell lineage from early Pro-B to mature B cells, but can also be found on activated T cells, LAK cells, NK progenitors, and T cells in lpr/lpr mutant mice.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Recommended: Titrate antibody for optimal performance

Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is designed for use with violet (405 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution. BV605 is not compatible with methanol-based fixation.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Cat. SB-4401-42) or Brilliant Stain Buffer™ (Cat. 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, regulating cell growth, differentiation, and signaling. CD45 is expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling. It exists in multiple isoforms due to alternative splicing, affecting expression and function. CD45 plays a key role in lymphocyte development, integrin-mediated signaling, and apoptosis regulation. Disorders associated with CD45 dysfunction include Severe Combined Immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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