Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 510 / 610-30S / 660-40S
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

The A20 monoclonal antibody reacts with mouse CD45 (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed on all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titration recommended for optimal performance.
• Fluorochrome: Brilliant Violet™ 605 (BV605) emits at 605 nm; use with violet (405 nm) laser.
• Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54); store up to 3 days at 4°C in dark. Not compatible with methanol-based fixation.
• Excitation / Emission / Laser: 407 nm / 605 nm / Violet laser

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, plays a role in lymphocyte development and antigen signaling, and modulates integrin-mediated pathways.
Alternative splicing produces multiple isoforms differing in extracellular domains, influencing cell-type-specific expression and membrane localization. CD45RA is one such isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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