Thermo Fisher Scientific Phospho-nNOS (Ser1417) Polyclonal Antibody
Phospho-nNOS (Ser1417) 특이적 폴리클로날 항체로, Western blot 및 면역형광에 적합. 인간 및 랫트 시료에서 인산화 nNOS 검출에 사용. 항원 친화 크로마토그래피로 정제된 고순도 항체. PBS/BSA 완충액에 보존, -20°C에서 안정적 저장 가능.
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- PA1032
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카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 9 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | View 1 publication |
| Immunoprecipitation (IP) | – | View 1 publication |
| Immunomicroscopy (IM) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325020 |
Product Specific Information
PA1-032 detects phosphorylated nNOS (Ser1417) from rat and human samples.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects an approximately 140 kDa protein representing recombinant rat phospho-nNOS (Ser1417).
The immunogen is a synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS.
The peptide sequence is 100% conserved in rat, frog, and mouse proteins.
This peptide (Cat. # PEP-188) is available for use in neutralization and control experiments.
Target Information
NOS (Nitric Oxide Synthase) activity requires homodimerization and three cosubstrates (L-arginine, NADPH, and O₂), as well as five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Three distinct NOS isoforms have been identified, encoded by separate genes:
- NOS1 (nNOS or ncNOS): Neuronal form, Ca²⁺/CaM-dependent
- NOS2 (iNOS): Inducible form
- NOS3 (eNOS or ecNOS): Endothelial form, Ca²⁺/CaM-dependent
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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