Thermo Fisher Scientific Phospho-nNOS (Ser1417) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:nNOS,uniProtId:P29475-1,ncbiNodeId:9606,antigenRange:1411-1425,antigenLength:1434,antigenImageFileName:PA1-032_nNOS_P29475-1_Rabbit.svg,antigenImageFileNamePDP:PA1-032_nNOS_P29475-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325020

Product Specific Information

PA1-032 detects phosphorylated nNOS (Ser 1417) from rat and human samples.

PA1-032 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~140 kDa protein representing recombinant rat phospho-nNOS (Ser 1417).

The PA1-032 immunogen is a synthetic phosphopeptide to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, frog, and mouse protein. This peptide (Cat. # PEP-188) is available for use in neutralization and control experiments.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.