Thermo Fisher Scientific iNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 69 publications 69 publications

Immunohistochemistry (IHC)

-

View 75 publications 75 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 4 publications 4 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50

View 15 publications 15 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Avian, Cat, Fish, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:iNOS,uniProtId:P29477-1,ncbiNodeId:10090,antigenRange:18-31,antigenLength:1144,antigenImageFileName:PA1-036_iNOS_P29477-1_Rabbit.svg,antigenImageFileNamePDP:PA1-036_iNOS_P29477-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325773

Product Specific Information

PA1-036 detects inducible nitric oxide synthase (iNOS) from human, mouse and rat tissues and cells as well as recombinant human and mouse iNOS. This antibody does not detect other NOS isoforms.

PA1-036 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse iNOS from LPS stimulated RAW 264.7 cells.

The PA1-036 immunogen is a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.