Thermo Fisher Scientific iNOS Polyclonal Antibody
인간, 생쥐, 랫트의 iNOS 단백질을 특이적으로 검출하는 Rabbit Polyclonal Antibody입니다. Western blot 및 Immunofluorescence에 적합하며 다른 NOS isoform과 교차 반응하지 않습니다. 합성 펩타이드 항원으로 제작되었으며 고순도 액상 형태로 제공됩니다.
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- PA1036
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Thermo Fisher Scientific iNOS Polyclonal Antibody (PA1-036)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | View 69 publications |
| Immunohistochemistry (IHC) | – | View 75 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | View 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50 | View 15 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunomicroscopy (IM) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Amphibian, Avian, Cat, Fish, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325773 |
Product Specific Information
PA1-036 detects inducible nitric oxide synthase (iNOS) from human, mouse, and rat tissues and cells, as well as recombinant human and mouse iNOS.
This antibody does not detect other NOS isoforms.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, it detects an ~135 kDa protein representing mouse iNOS from LPS-stimulated RAW 264.7 cells.
The immunogen is a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
Target Information
Inducible Nitric Oxide Synthase (iNOS) is an enzyme that produces nitric oxide (NO), a gaseous free radical acting as a cellular messenger.
NO mediates vasorelaxation, neurotransmission, and cytotoxicity.
iNOS expression is induced by lipopolysaccharides and cytokines in liver and other tissues.
It catalyzes oxidation of L-arginine to L-citrulline and NO.
Three NOS isoforms exist: neuronal (nNOS, type I), endothelial (eNOS, type III), and inducible (iNOS, type II).
iNOS is found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
Cytokines such as IFN-γ, TNF, IL-1, IL-2, and LPS upregulate iNOS expression and activity.
After induction, iNOS shows delayed activation followed by sustained NO production.
iNOS dysfunction is associated with diseases such as achalasia and impotence.
Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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