Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (12F4), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:1,000

-

Dot blot (DB)

1:1,000

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12F4

Immunogen

Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

A 1:1,000 dilution of MA5-45863 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Target Information

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an alpha,beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.