Himedia Antibiotic Assay Medium G

Antibiotic Assay Medium G

Details

This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis and is formulated in accordance to British Pharmacopoeia . It is also used for preparing the inoculum of Mycobacterim smegmatis for assay. To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid.