Moeller Decarboxylase Broth w/ Ornithine HCl
Details
Moeller Decarboxylase Broth w/ c HCl is used for differentiation of bacteria on the basis of their ability to decarboxylate L-Ornithine hydrochloride. Ornithine is decarboxylated to form putrescine. Formation of the amine putrescine increases the pH of the medium, changing the colour of the indicator from yellow to purple. Bromo cresol purple is the pH indicator. If the organisms do not produce the appropriate enzyme, the medium remains acidic, yellow in colour.Pseudomonas aeruginosa shows poistive Arginine decarboxylase activity, Salmonella and Shigella species shows delayed positive activity.
