Hemmes Medium Base
Details
Hemmes Medium Base is a screening medium for Salmonella and Shigella. TThe differentiation is based on seven reactions namely - dextrose, lactose, and sucrose fermentation, hydrogen sulphide production, urease detection, indole production and motility testing. Salmonella and Shigella show a red slant and a yellow butt from dextrose fermentation. Salmonella Typhimurium is motile, H2S positive indicated by blackening of the medium, indole negative and ureasenegative. Shigella are nonmotile, H2S negative, urease negative and indole negtaive.