Details
Tryptose Yeast Extract Broth (AOAC) with the addition of salicin or raffinose and an acid base indicator (Phenol Red) is recommended by AOAC for detection of Clostridium perfringens in food. This makes detection and isolation of these organisms from food important. Tryptose Yeast Extract Broth is recommended by AOAC for the confirmation of C. perfringens in foods. Clostridium perfringens produces a heat-resistance enterotoxin which causes food-poisoning if ingested. In perfringens poisoning, the vehicle is almost always an improperly cooked meat. The heat resistance of its spores often allows C. perfringens to survive incomplete cooking of food, with the surviving bacteria then able to cause food poisoning. This makes detection and isolation of these organisms from food important.
