Thermo Fisher Scientific ZONAB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

3-4 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

2-3 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Immunoprecipitation (IP)

1-2 µg

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Dog, Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of mouse ZONAB (ZO-1 associated nucleic acid-binding protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533460

Product Specific Information

This antibody reacts with an internal region of the ZONAB protein. On Western blots, it identifies a major band at ~65 kDa and a minor band at ~54 kDa. The antibody is directed towards a region of ZONAB common to both the long and the alternatively-spliced short form. During development, reactivity was confirmed using mouse testis tissue homogenates by Western blotting and labeling of mouse testis tissue using immunofluorescence. All signals were absent in knockout mice, further confirming the reactivity with ZONAB.

Target Information

Human DNA-binding protein (dbpA) is a member of a Y-box binding protein family containing a cold shock domain. The increased expression of Y box binding proteins in somatic cells is associated with cell proliferation and transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.